Improved gene expression in difficult to transfect cells with jetOPTIMUS® Transfection Reagent

FOLLOW BARIA

Best-in-class DNA transfection reagent jetOPTIMUS®

Race your DNA transfection in the cell model you need!

As Transfection Experts, we know that it is essential for biologists to work with physiologically relevant cell models.

For gene expression studies, transfection is the preferred technique to introduce a gene of interest in a given cell model, due to its cost-effectiveness and simplicity-of-use in comparison to physical techniques (e.g. micro-injection, electroporation).

Several types of cells (primary cells, stem cells or cancer cell lines) remain difficult to transfect with DNA for different reasons: slow-dividing rates, cell fragility and cellular defense mechanisms.

To address the current limits of DNA transfection and complement our existing gene expression portfolio (jetPEI®, jetPRIME®, jetMESSENGER®), Polyplus-transfection® engineered an innovative delivery nanoparticle: jetOPTIMUS®.

jetOPTIMUS® is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells (even in hard-to-transfect cells) resulting in higher transfection efficiency.

In order to work in relevant physiological conditions, transfection with jetOPTIMUS® requires a minimum DNA quantity and reagent volume to keep an excellent cell viability and morphology.

Specifications

Reagent

Molecule delivered

Applications

Cell types

Number of transfections

Storage

Provided with

jetOPTIMUS® DNA Transfection Reagent

Plasmid DNA

Transient and stable gene expression from plasmid DNA transfectionCRISPR Genome editing using DNA approach

Adherent hard-to-transfect mammalian cell:

- Primary cells (epithelial, hepatocyte, endothelial, fibroblast, etc…)

- Stem cells (embryonic, mesenchymal, induced pluripotent)

- Cancer cell lines

1.5 ml of jetOPTIMUS® transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol

5°C ± 3°C, stable for at least one year when stored appropriately

jetOPTIMUS® Buffer

Optimized DNA transfection efficiency

on hard-to-transfect cells

jetOPTIMUS® is an innovative cationic nanotechnology developed to improve DNA transfection efficiency in easy- and difficult-to-transfect cells used as in vitro cell culture models.

Tested on various primary cells and cell lines, jetOPTIMUS® proved its superiority by reaching higher transfection efficiencies and gene expression than main competitors.

jetOPTIMUS® outperforms its main competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection with a plasmid coding for a GFP protein in 24-well plates. Conditions were set up according to the manufacturer’s recommendations both for Lipofectamine® 3000 and for jetOPTIMUS®.

Advanced nanotechnology: Superior gene expression using less material!

jetOPTIMUS® was developed to increase the efficiency of transfection into a wide range of cell types using as little DNA as possible and to reduce the volume of reagent required in comparison to major competitors.

As a result, the number of transfections can be increased and response costs can be reduced by up to 7 times.

Table 1: Lower volume of jetOPTIMUS® is required compared to Lipofectamine® 3000 in 24-well plate.

Excellent cell viability and morphology

Reaching high transfection efficiencies is often at the expense of cellular integrity, with adverse effects on cell cycle, metabolism and signaling pathways.

At Polyplus-transfection, we make no compromise on cell integrity: jetOPTIMUS® transfection reagent is gentle on cells, allowing generation of biologically relevant data from gene expression studies.

Cells remains healthy and keep a good morphology 24h after transfection. Phase contrast microscopy of both MDCK and VERO cells 24 h after transfections with a plasmid coding for a GFP protein performed according to the manufacturer’s recommendations for each reagent.

Optimized transfection protocol

jetOPTIMUS® is a ready-to-use transfection reagent provided with its own complexation buffer (jetOPTIMUS® Buffer).

The protocol is optimized for simplicity of use (all plate sizes), culture medium compatibility (antibiotics, serum) and cost-effectiveness (lowest amount of DNA and volume of reagent).

Simplicity of the jetOPTIMUS® protocol for DNA transfection, here in 24-well plates.

Baria s.r.o. IČ: 26697904. DIČ: CZ26697904 Vídeňská 1764/158, 148 00 Praha 4 Tel:+420 244911228 E-mail:info@baria.cz